Comparison of Trichoderma longibrachiatum Xyloglucanase Production Using Tamarind (Tamarindus indica) and Jatoba (Hymenaea courbaril) Seeds: Factorial Design and Immobilization on Ionic Supports

Xyloglucan (XG) is the predominant hemicellulose in primary cell wall of superior plants. It has a fundamental role controlling stretching and expansion plant wall. There are five types enzymes known to cleave linear chain xyloglucan, most well-known xyloglucanase (XEG). The immobilization process can be used solve problems related stability, besides economic benefits brought by possibility its repeated use recovery. Therefore, this study aims at optimization production Trichoderma longibrachiatum using central composite rotatable design (CCRD) with tamarind jatoba seeds as carbon sources, well XEG on ionic supports, such MANAE (monoamine-N-aminoethyl), DEAE (diethylaminoethyl)-cellulose, CM (carboxymethyl)-cellulose, PEI (polyethyleneimine). High concentrations sources (1.705%), temperature 30 °C under agitation for 72 h, were favorable conditions activity from T. respect both sources. However, showed 23.5% higher compared seeds. source was chosen continue experiments. scaling up Erlenmeyer flasks bioreactor increased 1.27-fold (1.040 ± 0.088 U/mL). Regarding biochemical characterization crude extract, optimal range 50–55 °C, pH 5.0. stabilities temperature, not stable prolonged periods, which crucial immobilizing it resins. best efficiency CM-cellulose DEAE-cellulose, activities 1.16 0.89 U/g derivative (enzyme plus support), respectively. This describes, first time literature, fungal these supports.